Region

应用

Peptides from Serum on Strata™ X

样品制备详细信息

Part Number :8B-S100-TAK
Description :Strata-X 33 µm Polymeric Reversed Phase, 30 mg / 1 mL, Tubes, 100/Pk
Condition 1 :1.0 mL Methanol
Condition 2 :1.0 mL Water
Sample Pretreatment :Strata X 33u 30 mg / 1 ml Tube Condition: 1ml Methanol Load: 1 ml Spiked porcine serum extract
Load :Load 1.00 mL Sample
Wash 1 :1.0 mL 10% Acetonitrile in 0.1% TFA
Dry :1 min @ 10 in. Hg
Elution 1 :1.0 mL Acetonitrile:Water
Evaporate: :Evaporate to dryness
Reconstitute :300 µL TFA (0.1%) in Water
Notes :EVAPORATE AND RECONSTITUTE .40 °C under nitrogen stream 300 mL 0.1% aqueous TFA

应用详细信息 (应用ID: 14616)

货号:00F-4396-E0
尺寸:150 x 4.6 mm ID
洗脱类型:Gradient
洗脱A:0.1% TFA
洗脱B:ACN:0.1% TFA in water (90:10)
梯度程序:
步骤序号 时间 (分) 图片A 图片B
1 0 85 15
2 22 40 60

流速 (毫升/分钟):1 mL/min
冷却温度: 30 °C
检测: UV-Vis Abs.-Diode Array (PDA) @ 214 nm (22°C)
备注:SecurityGuard™ Guard Cartridge System extends column lifetime.
- SecurityGuard Cartridges, C12 4 x 3.0mm, 10/Pk Part No.: AJ0-6074
- Holder Part No.: KJ0-4282

分析物详细信息

分析物详细信息不可用

1
Oxytocin
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2
Angiotensin II
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3
Angiotensin I
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