Region

应用

Extraction of Barbiturates from Urine on Strata™ X

样品制备详细信息

Part Number :8B-S100-TAK
Description :Strata-X 33 µm Polymeric Reversed Phase, 30 mg / 1 mL, Tubes, 100/Pk
Condition 1 :1.0 mL Methanol
Condition 2 :1.0 mL Water
Load :Load 1.00 mL Sample diluted in KH2PO4 (100mM) pH 6.0
Wash 1 :1.0 mL Methanol / Water (5:95)
Wash 2 :1.0 mL Methanol / 2% CH3COOH in Water (10:90)
Elution 1 :1.0 mL Methanol / 2% Ammonium Hydroxide in Water (35:65)
Evaporate: :Evaporate to dryness
Reconstitute :300 µL Water
Notes :Blow down under slow stream of N2 @ 40° C and reconstitute with 300 µL water

应用详细信息 (应用ID: 14622)

货号:00F-4337-E0
尺寸:150 x 4.6 mm ID
洗脱类型:Gradient
洗脱A:20 mM KH2PO4, pH 7.0
洗脱B:Acetonitrile
梯度程序:
步骤序号 时间 (分) 图片A 图片B
1 0 80 20
2 3 80 20
3 15 50 50
4 16 80 20

流速 (毫升/分钟):1 mL/min
冷却温度: 室温
检测: UV-Vis Abs.-Fixed Wave. (UV) @ 210 nm (22°C)
备注:SecurityGuard™ Guard Cartridge System extends column lifetime.
- SecurityGuard Cartridges, C12 4 x 3.0mm, 10/Pk Part No.: AJ0-6074
- Holder Part No.: KJ0-4282

分析物详细信息

分析物详细信息不可用

1
Phenobarbital
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2
Butabarbital
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3
Butalbital
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4
Amobarbital
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5
Secobarbital
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