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应用

Benzodiazepines (SPE-Plasma) on Hybrid C18 Column

样品制备详细信息

Part Number :8B-S100-TAK
Description :Strata-X 33 µm Polymeric Reversed Phase, 30 mg / 1 mL, Tubes, 100/Pk
Condition 1 :1mL Methanol
Condition 2 :1mL Water
Sample Pretreatment :SPE: Strata X 30mg/ml Sample: 0.5ml human plasam + 0.5ml water + 200ng benzodiazepines Condition: 1ml MeOH, 1ml Water Washing: 1ml water, 1ml 40%MeOH in water Elution: 1ml MeOH Reconstitution: 100ul water
Load :Load 1.00 mL Sample diluted in Water
Wash 1 :1mL Water
Wash 2 :1mL 40% Methanol in Water
Elution 1 :1mL Methanol
Evaporate: :Evaporate to dryness
Reconstitute :100 µL Water

应用详细信息 (应用ID: 15454)

货号:00F-4435-Y0
尺寸:150 x 3 mm ID
洗脱类型:Gradient
洗脱A:0.1% Formic acid in Water
洗脱B:0.1% Formic acid in Acetonitrile
梯度程序:
步骤序号 时间 (分) 图片A 图片B
1 0 80 20
2 8 20 80

流速 (毫升/分钟):0.8 mL/min
冷却温度: 室温
检测: UV-Vis Abs.-Variable Wave.(UV) @ 254 nm (22°C)
备注:SecurityGuard™ Guard Cartridge System extends column lifetime.
- SecurityGuard Cartridges, Gemini C18 4 x 2.0mm, 10/Pk Part No.: AJ0-7596
- Holder Part No.: KJ0-4282

分析物详细信息

分析物详细信息不可用

1
Chlodiazepoxide
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2
Flurazepam
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3
Oxazepam
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4
Clonazepam
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5
Temazepam
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6
Diazepam
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