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Protein Precipatation vs. Ion Suppression of Medium Polar Drugs on strata Impact Square Well Plate

应用详细信息 (应用ID: 15712)

货号:00F-4439-Y0
尺寸:150 x 3 mm ID
洗脱类型:Gradient
洗脱A:0.1% Formic acid/Water
洗脱B:0.08% Formic acid/Acetonitrile
梯度程序:
步骤序号 时间 (分) 图片A 图片B
1 0 90 10
2 5 50 50
3 7 50 50

流速 (毫升/分钟):0.5 mL/min
冷却温度: 室温
检测: Mass Spectrometer (MS) @ 249 amu (22°C)
备注:Protein Precipitation Protocol:
Phase: Strata Impact Square Well Plate, 2 mL (CEO-7565)
1. Dispense 300 uL acetonitrile into each well using an automatic pipettor.
2. Place the protein precipitation plate onto a suitable 96-well vacuum manifold . Make sure that a 96-well collection plate is positioned inside the manifold to collect the filtrate.
3. Dispense 100 uL of Porcine plasma into each well ( acetonitrile:plasma = 3:1). Let it stand for 2 mins (no vortex /mixing required).
4. Apply 5-10" of mercury for 30-40 secs.
5. Collect the filtrate and blow down to dryness under slow stream of nitrogen @ 40 deg. C.
6. Reconstitute with 100 uL of mobile phase containing 10.0 ng of analye.
Note: For ion suppression or enhancement estimation, a set of 4 blank (100 uL of water instead of plasma) was run in parallel.

Observation:
Filtrate looked very clean and clear
Results:
Analyte logP m/z % Variation Effect
1. Pindolol 1.75 249 15% Suppression
2. Metoprolol 1.88 268 13% Suppression

分析物详细信息

分析物详细信息不可用

1
Pindolol
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2
Metoprolol
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