Region

应用

20nt DNA Oligo-RP Purification - 20 Minute Dry Out

样品制备详细信息

Part Number :8K-S042-FBR
Description :Clarity 70u QSP, 3mL/200mg Cartridge, 384/Pk
Condition 1 :1.5 mL Methanol/Water (1:1) at 1 mL/min
Condition 2 :6 mL Triophilic Buffer at 1 mL/min
Load :Load 1.50 mL Sample diluted in Triophilic Buffer
Wash 1 :8 mL Triophilic Buffer at 1 mL/min
Wash 2 :3 mL 2% TFA/50mM NaCl at 1 mL/min
Wash 3 :4 mL Water at 1 mL/min
Elution 1 :1 mL 40%Methanol/40%Water/20%Acetonitrile at 1 mL/min
Evaporate: :Evaporate to dryness

应用详细信息 (应用ID: 15805)

货号:00B-4441-E0
尺寸:50 x 4.6 mm ID
洗脱类型:Gradient
洗脱A:50mM TEAA/ 5%ACN
洗脱B:Methanol
梯度程序:
步骤序号 时间 (分) 图片A 图片B
1 0 90 10
2 20 40 60

流速 (毫升/分钟):1 mL/min
冷却温度: 室温
检测: UV-Vis Abs.-Variable Wave.(UV) @ 260 nm (22°C)
备注:Post conditioning 20 minute vacuum drying (compare with control sample AppID 15804)

分析物详细信息

分析物详细信息不可用

1
20 min Dry Out: DNA 20mer