Region

应用

1µmole DNA Cartridge Purification - Crude & Elution Fractions

样品制备详细信息

Part Number :8K-S042-FBR
Description :Clarity 70u QSP, 3mL/200mg Cartridge, 384/Pk
Condition 1 :1.5 mL 50% Methanol/ Water at 1 mL/min
Condition 2 :2 mL Clarity Triophilic 3:1 Buffer, 3 times of at 1 mL/min
Load :Load 1.00 mL Sample diluted in Clarity Triophilic 3:1 Buffer
Wash 1 :6 mL Clarity Triophilic 3:1 Buffer, 3 times of at 1 mL/min
Wash 2 :1.5 mL 2% TFA/50mM NaCl, 2 times of at 1 mL/min
Elution 1 :1 mL Methanol / Nuclease-free Water / Acetonitrile (40/40/20) at 1 mL/min
Evaporate: :Evaporate to dryness

应用详细信息 (应用ID: 15981)

货号:00B-4441-E0
尺寸:50 x 4.6 mm ID
洗脱类型:Gradient
洗脱A:50mM TEAA/5% Acetonitrile
洗脱B:Methanol
梯度程序:
步骤序号 时间 (分) 图片A 图片B
1 0 90 10
2 20 40 60

流速 (毫升/分钟):1 mL/min
冷却温度: 室温
检测: UV-Vis Abs.-Variable Wave.(UV) @ 260 nm (22°C)
备注:SecurityGuard™ Guard Cartridge System extends column lifetime.
- SecurityGuard Cartridges, Clarity Oligo-RP 4 x 3.0mm, 10/Pk Part No.: AJ0-8135
- Holder Part No.: KJ0-4282

分析物详细信息

分析物详细信息不可用

1
DNA
search