Region

应用

Simultaneous Desalting and Selective Elution of Basic, Neutral and Acidic Peptides

样品制备详细信息

Part Number :8B-S100-TAK
Description :Strata-X 33 µm Polymeric Reversed Phase, 30 mg / 1 mL, Tubes, 100/Pk
Condition 1 :1 mL Acetonitrile
Condition 2 : Water
Load :Load 840.00 µL Sample diluted in water with salt
Wash 1 :2 mL Water
Elution 1 :0.25 mL 40:60 Acetonitrile/Water. for acidic peptides
Elution 2 :0.25 mL 70:30 Acetonitrile/Water. for neutral peptides
Elution 3 :0.25 mL 70:30 Acetonitrile/1.0% Formic Acid in Water. for basic peptides
Evaporate: :Evaporate to dryness
Reconstitute :20 µL initial mobile phase

应用详细信息 (应用ID: 16087)

货号:00F-4337-E0
尺寸:150 x 4.6 mm ID
洗脱类型:Gradient
洗脱A:0.1% TFA in Water
洗脱B:0.1% TFA in Acetonitrile
梯度程序:
步骤序号 时间 (分) 图片A 图片B
1 0 90 10
2 12 35 65

流速 (毫升/分钟):1 mL/min
冷却温度: 室温
检测: UV-Vis Abs.-Variable Wave.(UV) @ 214 nm (22°C)
备注:SecurityGuard™ Guard Cartridge System extends column lifetime.
- SecurityGuard Cartridges, C12 4 x 3.0mm, 10/Pk Part No.: AJ0-6074
- Holder Part No.: KJ0-4282

分析物详细信息

分析物详细信息不可用

1
Adrenocorticotropin Hormone Fragment 1-24 (ACTH)
search

2
Bradykinin
search

3
Methionine enkephalin
search

4
Gonadotropin Releasing Hormone (HGRH) Fragment 14-26