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应用

Nicotine and Metabolites on Gemini 5µm C18

应用详细信息 (应用ID: 16345)

货号:00D-4435-B0
尺寸:100 x 2 mm ID
洗脱类型:Gradient
洗脱A:Water with 0.1% v/v Formic Acid
洗脱B:Acetonitrile with 0.1% v/v Formic Acid
梯度程序:
步骤序号 时间 (分) 图片A 图片B
1 0 97 3
2 1 97 3
3 3 70 30
4 3.01 97 3
5 5.5 97 3

流速 (毫升/分钟):0.4 mL/min
冷却温度: 室温
检测: Mass Spectrometer (MS) @ amu (22°C)
检测器信息: SCIEX API 3000™ Triple Quadrupole Mass Spectrometer with TurboIonSpray Source
MRM transitions monitored:
Nicotine: m/z 163.3/105.9 and 163.3/80.1
Nornicotine: m/z 149.1/80.1
Cotinine: m/z 177.3/98.0 and 177.3/80.1
备注:The pH of the aqueous acid is 2.7. 0.5ng on-column injection of each analyte.

分析物详细信息

分析物详细信息不可用

1
Nornicotine
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2
Nicotine
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3
Cotinine
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