Region

应用

PEGylated proteins on Jupiter® 300 C18 3µm and 5µm, and C4 5µm columns

应用详细信息 (应用ID: 17521)

货号:00F-4263-E0
尺寸:150 x 4.6 mm ID
洗脱类型:Gradient
洗脱A:0.1% TFA, 2% ACN in Water
洗脱B:90% ACN, 0.085% TFA in Water
梯度程序:
步骤序号 时间 (分) 图片A 图片B
1 0 80 20
2 25 35 65

流速 (毫升/分钟):1 mL/min
冷却温度: 45 °C
检测: UV-Vis Abs.-Variable Wave.(UV) @ 214 nm (25°C)
备注:Compare reverse phase separations of different PEGylated and intact proteins selected at different reaction time-points on three different columns. (A) PEGylated alpha-Chymotrypsinogen A with PEG1 Amine 20kDa Methoxy PEG NHS-ester, 0 min fraction; (B) PEGylated alpha-Chymotrypsinogen A with PEG3 N-Terminus 20kDa Methoxy PEG aldehyde, 120 min fraction; (C) PEGylated beta-Lactoglobulin A with PEG4 N-Terminus 40kDa Y-shape Methoxy PEG aldehyde, overnight fraction. Well-noticed, good resolution was achieved on Jupiter 3µm C18 small particles versus larger Jupiter 5µm C18 particles or Jupiter C4 bonded media.

分析物详细信息

分析物详细信息不可用

1
PEGylated Proteins