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应用

Column Diameter Study with Luna 5u C18(2) 50 x 21.2mm

应用详细信息 (应用ID: 18063)

尺寸:50 x 21.2 mm ID
洗脱类型:Gradient
洗脱A:0.5% TFA in Acetonitrile
洗脱B:0.5% TFA in Water
梯度程序:
步骤序号 时间 (分) 图片A 图片B
1 0 5 95
2 5 95 5

流速 (毫升/分钟):30 
冷却温度: 室温
检测: UV-Vis Abs.-Variable Wave.(UV) @ 254 nm (22°C)
备注:Comparision of column diameter on preparative separations using Axia-packed columns in various diameters: 4.6 (conventional hardware), 21.2, 30 and 50mm (cf: App IDs: 18062 - 18065).
Load varied by diameter as follows:
- 4.6mm: 64 µg in 21 µL DMSO (scaling factor = 1)
- 21.2mm: 1.2 mg in 420 µL DMSO (scaling factor = 20)
- 30mm: 2.4 mg in 840 µL DMSO (scaling factor = 40)
- 50mm: 7.3 mg in 2100 µL DMSO (scaling factor = 118)

分析物详细信息

分析物详细信息不可用

1
Propranolol
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2
Diphenhydramine
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