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应用

Poly dT 12-18 standard on Oligo-RP (modifying TEA/HFIP Ratio)

应用详细信息 (应用ID: 18089)

货号:00F-4441-B0
尺寸:150 x 2 mm ID
洗脱类型:Gradient
洗脱A:TEAA/HFIP/2%MeOH in Water
洗脱B:Acetonitrile
梯度程序:
步骤序号 时间 (分) 图片A 图片B
1 0 100 0
2 10 90 10
3 30 70 30

流速 (毫升/分钟):0.2 mL/min
冷却温度: 室温
检测: Mass Spectrometer (MS) @ 400 amu (22°C)
备注:Different ratios of HFIP to TEA were investigated using the poly dT 12-18 standard mixture. HFIP was fixed at 200 mM and different levels of TEA were used between 1 and 20 mM (4mm-red trace {pH 7.6}, 8mm-blue trace{pH8.0} and 14mm -black trace{pH8.3}). Optimal retention, MS response, and resolution were observed for the 200 mM HFIP./ 8mM TEA mobile phase mixture.

分析物详细信息

分析物详细信息不可用

1
Poly dT 12-18 Oligonucleotide
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