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应用

Metanephrine and Normetanephrine on Luna HILIC 3um 50X2.0mm

应用详细信息 (应用ID: 18291)

货号:00B-4449-B0
尺寸:50 x 2 mm ID
洗脱类型:Gradient
洗脱A:95/5 ACN/100 mM ammonium formate, pH 3.2
洗脱B:50/45/5 ACN/Water/100 mM ammonium formate, pH 3.2
梯度程序:
步骤序号 时间 (分) 图片A 图片B
1 0 100 0
2 2.5 100 0
3 2.51 0 100
4 3 0 100
5 3.01 100 0

流速 (毫升/分钟):0.4 mL/min
冷却温度: 室温
检测: Mass Spectrometer (MS) @ (22°C)
备注:LC/MS/MS & Chromatographic Condition
MS Detector: API 3000 LC/MS/MS with ESI (TurboIonSpray®) (www.Appliedbiosystems.com), Positive Ion Mode; MRM; heater gas flow 7000 cc/min; heater temperature 550 °C; Column: LUNA 3µm HILIC, 50x2.0mm; Flow rate: 0.4ml/min
ESI Source Conditions:
NEB: 9.00
CUR: 10.00
IS: 3500.00
TEM: 550.00
CAD: 11.00
CE 23.00 Normetanephrine
CE 25.00 Metanephrine
Analyte Q1 Q3 Time DP FP CE CXP
NMN 166.4 134.2 150 61 370 23 8
MN 180.4 148.2 150 71 370 25 8

分析物详细信息

分析物详细信息不可用

1
Metanephrine
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2
Normetanephrine
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