Region

应用

PCP on Kinetex 2.6µm C18 50 x 2.1mm ID by LCMS

样品制备详细信息

Part Number :8B-S029-TBJ
Description :Strata-X-C 33 µm Polymeric Strong Cation, 30 mg / 3 mL, Tubes, 50/Pk
Condition 1 :1 mL methanol at 1 mL/min
Condition 2 :1 mL water at 1 mL/min
Load :Load 1.00 mL Sample diluted in 1
Wash 1 :1 mL 0.1N hydrochloric acid at 2 mL/min
Wash 2 :1 mL Methanol at 2 mL/min
Elution 1 :0.5 mL 5% NH4OH/methanol at 1 mL/min
Elution 2 :0.5 mL 5% NH4OH/methanol at 1 mL/min
Evaporate: :Evaporate to dryness
Reconstitute :200 µL Initial LC mobile phase (97/3)

应用详细信息 (应用ID: 18644)

货号:00B-4462-AN
尺寸:50 x 2.1 mm ID
洗脱类型:Gradient
洗脱A:0.1% Formic acid in H2O
洗脱B:0.1% Formic acid in ACN
梯度程序:
步骤序号 时间 (分) 图片A 图片B
1 0 97 3
2 0.25 97 3
3 2.75 5 95
4 3 5 95
5 3.01 97 3
6 4.5 97 3

流速 (毫升/分钟):500 µL/min
冷却温度: 25 °C
检测: Mass Spectrometer (MS) @ amu (22°C)
备注:SecurityGuard™ ULTRA Guard Cartridge System extends column lifetime.
- SecurityGuard ULTRA Cartridges, UHPLC C18 for 2.1mm ID Columns, 3/Pk Part No.: AJ0-8782
- Holder Part No.: AJ0-9000

分析物详细信息

分析物详细信息不可用

1
PCP
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2
PCP-d5
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