Region

应用

Clarity OTX- MS Sensitivity of recovered 21mer at 500ng/mL from plasma

样品制备详细信息

Part Number :8E-S103-EGA
Description :Clarity OTX, 100 mg / well, 96-Well Plates, Ea
Condition 1 :0.5 mL MeOH at 1 mL/min
Condition 2 :0.5 mL Clarity OTX Equilibration Buffer at 1 mL/min
Load :Load 500.00 µL Sample diluted in OTX loading buffer/sample
Wash 1 :0.5 mL Clarity OTX Wash Buffer at 1 mL/min
Wash 2 :0.5 mL Clarity OTX Equilibration Buffer at 1 mL/min
Dry :10 um vaccuum
Elution 1 :0.5 mL Clarity OTX Elution Buffer at 1 mL/min
Evaporate: :Evaporate to dryness
Reconstitute :200 µL Mobile phase A

应用详细信息 (应用ID: 18870)

货号:00B-4441-B0
尺寸:50 x 2 mm ID
洗脱类型:Gradient
洗脱A:1%HFIPA/ 0.1%DIEA/ water
洗脱B:90% MeOH/ 0.075%HFIPA/ 0.0375% DIEA
梯度程序:
步骤序号 时间 (分) 图片A 图片B
1 0 90 10
2 20 65 35

流速 (毫升/分钟):0.25 mL/min
冷却温度: 60 °C
检测: Mass Spectrometer (MS) @ amu (22°C)
备注:SecurityGuard™ Guard Cartridge System extends column lifetime.
- SecurityGuard Cartridges, Clarity Oligo-RP 4 x 2.0mm ID, 10/Pk Part No.: AJ0-8134
- Holder Part No.: KJ0-4282

分析物详细信息

分析物详细信息不可用

1
21mer DNA oligo