Region

应用

Clarity OTX- Sensitivity of 19mer P-S Oligo from Liver Tissue

样品制备详细信息

Part Number :8E-S103-EGA
Description :Clarity OTX, 100 mg / well, 96-Well Plates, Ea
Condition 1 :0.5 mL MeOH at 1 mL/min
Condition 2 :0.5 mL Clarity OTX Equlibration Buffer at 1 mL/min
Sample Pretreatment :O
Load :Load 500.00 µL Sample diluted in 1:1 Clarity OTX loading buffer/ sample
Wash 1 :0.5 mL Clarity OTX wash solvent at 1 mL/min
Wash 2 :0.5 mL Clarity OTX equilibration Buffer at 1 mL/min
Dry :N2 at 10u vaccuum
Elution 1 :0.5 mL Clarity OTX elution buffer at 1 mL/min
Evaporate: :Evaporate to dryness
Reconstitute :100 µL Aobile phase A

应用详细信息 (应用ID: 18872)

货号:00B-4441-B0
尺寸:50 x 2 mm ID
洗脱类型:Gradient
洗脱A:200mM HFIP/ 14mM TEA/ 2% MeOH/ water
洗脱B:200mM HFIP/ 14mM TEA/ 98% MeOH/ water
梯度程序:
步骤序号 时间 (分) 图片A 图片B
1 0 90 10
2 10 35 65

流速 (毫升/分钟):0.25 mL/min
冷却温度: 40 °C
检测: UV-Vis Abs.-Variable Wave.(UV) @ 260 nm (22°C)
备注:SecurityGuard™ Guard Cartridge System extends column lifetime.
- SecurityGuard Cartridges, Clarity Oligo-RP 4 x 2.0mm ID, 10/Pk Part No.: AJ0-8134
- Holder Part No.: KJ0-4282

分析物详细信息

分析物详细信息不可用

1
19 mer P-S oligonucleotide