Region

应用

Extraction of Indole 3 carboxylic acid from Strata-X-A on Gemini 5u, C18, 150x4.6mm Column

样品制备详细信息

Part Number :8B-S123-TAK
Description :Strata-X-A 33 µm Polymeric Strong Anion, 30 mg / 1 mL, Tubes, 100/Pk
Condition 1 :1 mL Methanol at 1 mL/min
Condition 2 :1 mL Water at 1 mL/min
Load :Load 500.00 µL Sample diluted in Water
Wash 1 :1 mL 50 mm Na acetate, PH 7.0 at 1 mL/min
Wash 2 :1 mL Methanol at 1 mL/min
Dry :2 mins at 10" of Hg
Elution 1 :1000 mL 5% FA/MeOH at 1 mL/min
Evaporate: :Evaporate to dryness
Reconstitute :500 µL 20mm KH2PO4(PH 2.5)/ ACN=75/25
Notes :Elution solvent was applied in 2 aliquots of 500uL followed by evaporation to dryness at 45 deg C under N2 and reconstitute with 500 uL initial mobile phase (20mm KH2PO4(PH 2.5)/ ACN=75/25) spiked with 50ug/mL Salicylic acid (IS)

应用详细信息 (应用ID: 19274)

货号:00F-4435-E0
尺寸:150 x 4.6 mm ID
洗脱类型:Isocratic
洗脱A:20 mM KH2PO4(pH 2.5)
洗脱B:Acetonitrile
梯度程序:
步骤序号 时间 (分) 图片A 图片B
1 0 75 25

流速 (毫升/分钟):1 mL/min
冷却温度: 室温
检测: UV-Vis Abs.-Variable Wave.(UV) @ 220 nm (22°C)
备注:SecurityGuard™ Guard Cartridge System extends column lifetime.
- SecurityGuard Cartridges, Gemini C18 4 x 3.0mm ID, 10/Pk Part No.: AJ0-7597
- Holder Part No.: KJ0-4282

分析物详细信息

分析物详细信息不可用

1
Indole-3-carboxylic acid
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2
Salicylic acid
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