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应用

25-OH Vitamin D2 and D3 Standards from Serum on Fully Porous 5µm C18 after PPT

样品制备详细信息

Part Number :8B-S100-TBJ
Description :Strata-X 33 µm Polymeric Reversed Phase, 30 mg / 3 mL, Tubes, 50/Pk
Condition 1 :1 mL Acetonitrile
Condition 2 :1 mL Acetonitrile/Water (65:35)
Load :Load 1.60 mL Sample diluted in Acetonitrile/Water (50:50)
Wash 1 :0.5 mL Acetonitrile/Water (65:35) at 1 mL/min
Wash 2 :0.5 mL Acetonitrile/Water (65:35) at 1 mL/min
Elution 1 :0.5 mL Acetonitrile at 1 mL/min
Elution 2 :0.5 mL Acetonitrile at 1 mL/min
Evaporate: :Evaporate to dryness
Reconstitute :100 µL ethyl acetate/acetonitrile (5:95)
Notes :Note: Avoid over drying eluates to avoid loss of analytes and/or internal standard, which will impact both precision and accuracy of data Reconstitute with 100 µL ethyl acetate/acetonitrile (5:95), and vortex mix for 5 seconds Centrifuge for 10 min at 2000g to settle any precipitate Transfer clear liquid to amber-colored autosampler vial and cap

应用详细信息 (应用ID: 19315)

货号:00B-4252-B0
尺寸:50 x 2 mm ID
洗脱类型:Gradient
洗脱A:0.05 % Formic acid
洗脱B:5 mM Ammonium acetate + 0.1 % Formic acid in methanol
梯度程序:
步骤序号 时间 (分) 图片A 图片B
1 0 92 8
2 5 92 8
3 205 0 100
4 290 0 100
5 360 92 8

流速 (毫升/分钟):0.9 mL/min
冷却温度: 35 °C
检测: Mass Spectrometer (MS) @ amu (22°C)
检测器信息: SCIEX API 4000™
备注:SecurityGuard™ Guard Cartridge System extends column lifetime.
- SecurityGuard Cartridges, C18 4 x 2.0mm ID, 10/Pk Part No.: AJ0-4286
- Holder Part No.: KJ0-4282

分析物详细信息

分析物详细信息不可用

1
25-Hydroxy vitamin D2 (25-OH D2)
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2
25-Hydroxy vitamin D3-d6 (25-OH D3-d6)

3
25-Hydroxy vitamin D2 (25-OH D2)
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