Region

应用

0.1 ng/g Chloramphenicol in Shrimp using Strata-X and Kinetex C18 50 x 2.1 mm by LCMS

样品制备详细信息

Part Number :8B-S100-UBJ
Description :Strata-X 33 µm Polymeric Reversed Phase, 60 mg / 3 mL, Tubes, 50/Pk
Condition 1 :2 mL Methanol at 2 mL/min
Condition 2 :2 mL Water at 2 mL/min
Load :Load 10.00 mL Sample diluted in Water
Wash 1 :2 mL Water at 2 mL/min
Dry :5min @ >10 in Hg
Elution 1 : Ethyl Acetate
Evaporate: :Evaporate to dryness
Reconstitute :500 µL 20% ACN in water

应用详细信息 (应用ID: 19453)

货号:00B-4462-AN
尺寸:50 x 2.1 mm ID
洗脱类型:Gradient
洗脱A:5 mM Ammonium bicarbonate
洗脱B:Acetonitrile
梯度程序:
步骤序号 时间 (分) 图片A 图片B
1 0 95 5
2 2 95 5
3 2.01 5 95
4 4.5 5 95

流速 (毫升/分钟):0.4 mL/min
冷却温度: 室温
检测: Mass Spectrometer (MS) @ (450°C)
检测器信息: SCIEX API 4000™
备注:Extracted ion chromatogram for the lowest level (0.1 ng/mL or 0.01 ng/g of shrimp tissue) of extracted standard of chloramphenicol (321.2/152; top) and internal standard (326/157; bottom). The impurity peak eluting at about 3.0 minutes is well-resolved from chloramphenicol and does not affect quantitation.

分析物详细信息

分析物详细信息不可用

1
Chloramphenicol
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2
Chloramphenicol-d5
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3
impurity