Region

应用

Phencyclidine from urine using Strata-X-Drug B and Kinetex C18, 50 x 2.1 mm by LCMS

样品制备详细信息

Part Number :8B-S128-UCH
Description :Strata-X-Drug B 33 µm Polymeric Strong Cation, 60 mg / 6 mL, Tubes, 30/Pk
Condition 1 :2 at 2
Condition 2 :2 at 2
Load :Load 4.00 mL Sample diluted in Pre-treated Sample
Wash 1 : 2mL of 100mM Sodium acetate buffer (pH 5.0)
Wash 2 : 2mL of Methanol
Dry :10min @ >10 in Hg
Elution 1 : 2mL of Ethyl Acetate: IPA:NH4OH (7:2:1)
Evaporate: :Evaporate to dryness
Reconstitute :1 mL 20% methanol in water
Notes :Evaporate eluant to dryness and reconstitute in 1 mL of 20% methanol in water. Phencyclidine was spiked into the urine at the 25ng/mL cutoff.

应用详细信息 (应用ID: 19595)

货号:00B-4462-AN
尺寸:50 x 2.1 mm ID
洗脱类型:Gradient
洗脱A:0.1% Formic acid
洗脱B:Methanol with 0.1% Formic Acid
梯度程序:
步骤序号 时间 (分) 图片A 图片B
1 0 75 25
2 1 5 95
3 2 5 95
4 2.1 75 25
5 4 75 25

流速 (毫升/分钟):400 µL/min
冷却温度: 室温
检测: Mass Spectrometer (MS) @ (500°C)
备注:Source temp: 500ºC
Polarity: Positive
Dwell: 100 msec

分析物详细信息

分析物详细信息不可用

1
Phencyclidine-d5
search

2
Phencyclidine
search