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应用

Ibuprofen by HPLC for Kinetex 2.6um XB-C18, 75x4.6mm

应用详细信息 (应用ID: 19660)

货号:00C-4496-E0
尺寸:75 x 4.6 mm ID
洗脱类型:Isocratic
洗脱A:600:400 Acetonitrile: Water with 4g of chloroacetic acid adjusted to pH 3.0 with ammonium hydroxide
梯度程序:
步骤序号 时间 (分) 图片A
1 10 100

流速 (毫升/分钟):2 mL/min
冷却温度: 30 °C
检测: UV-Vis Abs.-Variable Wave.(UV) @ 254 nm (22°C)
备注:The USP monograph specifies two system suitability requirements that must be met; these requirements are resolution and tailing factor: resolution between ibuprofen and valerophenone (the internal standard), and valerophenone and ibuprofen related compound C must not be less than 2.5; tailing factor for each peak must not be more than 2.5.
On the Kinetex 2.6um XB-C18 75x4.6mm column at a flow rate of 2.0mL/min
Rs between ibuprofen and valerophenone is 4.65
Rs between valerophenone and ibuprofen related compound C is 4.67; and Tf for ibuprofen, valerophenone, and ibuprofen related compound C are 1.71, 1.02 and 0.999, respectively

分析物详细信息

分析物详细信息不可用

1
Ibuprofen
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2
Valerophenone
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3
Ibuprofen related compound C