Region

应用

Estrone, Estradiol, and Estriol from Water @ pH 2.0 Strata-X via Kinetex XB-C18 150x4.60mm

样品制备详细信息

Part Number :8B-S100-FCH
Description :Strata-X 33 µm Polymeric Reversed Phase, 200 mg / 6 mL, Tubes, 30/Pk
Condition 1 :5 mL Acetonitrile at 2 mL/min
Condition 2 :5 mL Methanol at 2 mL/min
Condition 3 :10 mL MilliQ Water at 2 mL/min
Load :Load 100.00 mL Sample diluted in Sample
Wash 1 : Not Required
Dry :1 Minute
Elution 1 :10 mL Acetonitrile at 2 mL/min
Evaporate: :Evaporate to dryness
Reconstitute :1 mL Methanol

应用详细信息 (应用ID: 19680)

货号:00F-4496-E0
尺寸:150 x 4.6 mm ID
洗脱类型:Gradient
洗脱A:6.9mM Ammonium Acetate pH 4.0 with 25% Acetonitrile (v/v)
洗脱B:Acetonitrile
梯度程序:
步骤序号 时间 (分) 图片A 图片B
1 0 100 0
2 10 35 65

流速 (毫升/分钟):1.2 mL/min
冷却温度: 室温
检测: UV-Vis Abs.-Variable Wave.(UV) @ 230 nm (22°C)
备注:SecurityGuard™ ULTRA Guard Cartridge System extends column lifetime.
- SecurityGuard ULTRA Cartridges, UHPLC C18 for 4.6mm ID Columns, 3/Pk Part No.: AJ0-8768
- Holder Part No.: AJ0-9000

分析物详细信息

分析物详细信息不可用

1
Estriol
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2
Ethyl paraben
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3
17-beta-Estradiol
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4
Estrone
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