Region

应用

LC/MS/MS Analysis of Digoxin and Digitoxin in plasma (0.25 ng/mL) using Strata-X and Kinetex C8

样品制备详细信息

Part Number :8B-S100-TBJ
Description :Strata-X 33 µm Polymeric Reversed Phase, 30 mg / 3 mL, Tubes, 50/Pk
Condition 1 :2 mL Methanol at 2 mL/min
Condition 2 :2 mL 10mM Ammonium acetate at 2 mL/min
Load :Load 3.00 mL Sample diluted in Water
Wash 1 :1 mL 50:50 10mM ammonium acetate/10mM ammonium acetate in methanol at 2 mL/min
Dry :10min at full vacuum
Elution 1 :2 mL Methanol at 1 mL/min
Evaporate: :Evaporate to dryness
Reconstitute :100 µL 50:50 Methanol/water

应用详细信息 (应用ID: 19691)

货号:00B-4497-AN
尺寸:50 x 2.1 mm ID
洗脱类型:Gradient
洗脱A:10mM Ammonium acetate
洗脱B:10mM Ammonium acetate in methanol
梯度程序:
步骤序号 时间 (分) 图片A 图片B
1 0 50 50
2 2.5 0 100
3 2.51 50 50
4 5 50 50

流速 (毫升/分钟):400 µL/min
冷却温度: 30 °C
检测: Mass Spectrometer (MS) @ (350°C)
检测器信息: SCIEX API 4000™
备注:SecurityGuard™ ULTRA Guard Cartridge System extends column lifetime.
- SecurityGuard ULTRA Cartridges, UHPLC C8 for 2.1mm ID Columns, 3/Pk Part No.: AJ0-8784
- Holder Part No.: AJ0-9000

分析物详细信息

分析物详细信息不可用

1
Digoxin
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2
Oleandrin
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3
Digitoxin
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