Region

应用

LC/MS/MS Analysis of Methadone from urine using Strata-X-Drug B on a Kinetex C18 2.6µm, 50x2.1mm

样品制备详细信息

Part Number :8B-S128-UCH
Description :Strata-X-Drug B 33 µm Polymeric Strong Cation, 60 mg / 6 mL, Tubes, 30/Pk
Load :Load Sample
Wash 1 : 2 mLs of 100mM sodium acetate buffer (pH 5)
Wash 2 : 2 mLs of Methanol
Dry :10 minutes @ full vacuum
Elution 1 : 2mLs of Ethyl acetate:Isopropanol:NH4OH (7:2:1)
Evaporate: :Evaporate to dryness
Reconstitute :1 mL 40% acetonitrile in water
Notes :Evaporate to dryness under a stream of nitrogen at 50 C . Reconstitute in 1 mL of 40% acetonitrile.

应用详细信息 (应用ID: 19703)

货号:00B-4462-AN
尺寸:50 x 2.1 mm ID
洗脱类型:Gradient
洗脱A:0.1% Formic acid
洗脱B:0.1% Formic acid in acetonitrile
梯度程序:
步骤序号 时间 (分) 图片A 图片B
1 0 60 40
2 3.5 0 100
3 3.51 60 40
4 6.5 60 40

流速 (毫升/分钟):400 µL/min
冷却温度: 室温
检测: Mass Spectrometer (MS) @ amu (22°C)
检测器信息: SCIEX API 3000™

Source: Cur-11, CAD-12, IS-4500, Tem-450, NEB-12
Dwell: 25ms
Polarity: Positive
备注:SecurityGuard™ ULTRA Guard Cartridge System extends column lifetime.
- SecurityGuard ULTRA Cartridges, UHPLC C18 for 2.1mm ID Columns, 3/Pk Part No.: AJ0-8782
- Holder Part No.: AJ0-9000

分析物详细信息

分析物详细信息不可用

1
Methadone-d9
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2
Methadone
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