Region

应用

Aldosterone on Kinetex 2.6 um XB-C18 50x3 mm ID

样品制备详细信息

Part Number :8B-S123-UBJ
Description :Strata-X-A 33 µm Polymeric Strong Anion, 60 mg / 3 mL, Tubes, 50/Pk
Condition 1 :3 mL Methanol
Condition 2 :3 mL 25 mM Ammonium bicarbonate
Load :Load 1.50 mL Sample diluted in 25 mM Ammonium bicarbonate (pH 8.9-9.0)
Wash 1 :3 mL 25 mM Ammonium bicarbonate
Wash 2 :3 mL 25% Methanol in water
Dry :5 min at high vacuum
Elution 1 :1 mL Ethyl acetate/Isopropanol/Ammonium hydroxide (7:2:1)
Elution 2 :1 mL Ethyl acetate/Isopropanol/Ammonium hydroxide (7:2:1)
Evaporate: :Evaporate to dryness
Reconstitute :100 µL Methanol/Water (30:70)

应用详细信息 (应用ID: 20009)

货号:00B-4496-Y0
尺寸:50 x 3 mm ID
洗脱类型:Gradient
洗脱A:DI Water
洗脱B:Methanol/Acetonitrile (50:50)
梯度程序:
步骤序号 时间 (分) 图片A 图片B
1 0 70 30
2 4 10 90
3 5 10 90
4 5.1 70 30
5 7 70 30

流速 (毫升/分钟):500 µL/min
冷却温度: 室温
检测: Tandem Mass Spec (MS-MS) @ (22°C)
检测器信息: SCIEX API 5000™
备注:SecurityGuard™ ULTRA Guard Cartridge System extends column lifetime.
- SecurityGuard ULTRA Cartridges, UHPLC C18 for 3.0mm ID Columns, 3/Pk Part No.: AJ0-8775
- Holder Part No.: AJ0-9000

分析物详细信息

分析物详细信息不可用

1
Aldosterone
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2
Cortisone
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