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应用

Phospholipid Removal Phree vs. Traditional PPT(Zoomed Scale)

样品制备详细信息

Part Number :8E-S133-TGB
Description :Phree Phospholipid Removal, 30 mg / well, 96-Well Plates, 2/Pk
Load :Load Sample
Evaporate: :Evaporate to dryness
Reconstitute :0 µL initial mobile phase

应用详细信息 (应用ID: 20012)

货号:00B-4462-AN
尺寸:50 x 2.1 mm ID
洗脱类型:Gradient
洗脱A:0.1% Formic Acid in Water
洗脱B:0.1% Formic Acid in Methanol
梯度程序:
步骤序号 时间 (分) 图片A 图片B
1 0 40 60
2 0.5 5 95
3 15.5 5 95
4 15.51 40 60
5 19.5 40 60

流速 (毫升/分钟):400 µL/min
冷却温度: 室温
检测: Mass Spectrometer (MS) @ 184 amu (425°C)
备注:Extracted sample was prepped with Phenomenex Phree Phospholipid Removal Plate. Step 1: Add 300uL of 1% formic acid in acetonitrile directly to Phree plate. Step 2: Add 100uL of plasma directly to Phree plate. Vortex and extract. This was compared to standard protein precipitated plasma using the same volume of plasma and crash solvent

分析物详细信息

分析物详细信息不可用

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Phospholipid Profile