Region

应用

Underivatized MMA and Succinic Acid in Human Urine on Gemini C18, 3u, 100x3 mm

样品制备详细信息

Part Number :8B-S038-TAK
Description :Strata-X-AW 33 µm Polymeric Weak Anion, 30 mg / 1 mL, Tubes, 100/Pk
Condition 1 :1 mL Methanol
Condition 2 :1 mL 25 mM Ammonium Formate
Load :Load Sample
Wash 1 :0.5 mL Methanol/Water (50:50)
Dry :Dry under high vacuum for 5-10 min
Elution 1 :0.6 mL Ammonium hydroxide/Methanol (2:98)
Elution 2 :0.6 mL Ammonium hydroxide/Methanol (2:98)
Evaporate: :Evaporate to dryness
Reconstitute :200 µL 0.1% Formic acid
Notes :This method is for the analysis of underivatized MMA.

应用详细信息 (应用ID: 20586)

货号:00D-4439-Y0
尺寸:100 x 3 mm ID
洗脱类型:Gradient
洗脱A:0.1% Formic Acid in DI H2O
洗脱B:0.1% Formic Acid + 10 mM Ammonium Formate in MeOH
梯度程序:
步骤序号 时间 (分) 图片A 图片B
1 0 85 15
2 1.5 5 95
3 2.5 5 95
4 2.51 85 15
5 4.5 85 15

流速 (毫升/分钟):700 µL/min
冷却温度: 40 °C
检测: Tandem Mass Spec (MS-MS) @ (22°C)
检测器信息: SCIEX API 4000™ (Negative Mode ESI)
备注:SecurityGuard™ Guard Cartridge System extends column lifetime.
- SecurityGuard Cartridges, Gemini C18 4 x 2.0mm ID, 10/Pk Part No.: AJ0-7596
- Holder Part No.: KJ0-4282

分析物详细信息

分析物详细信息不可用

1
Succinic acid
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2
Methylmalonic acid
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