Region

应用

Aldosterone analysis by LCMS using Kinetex 2.6um XB-C18 50x3.0mm

样品制备详细信息

Part Number :8B-S123-UBJ
Description :Strata-X-A 33 µm Polymeric Strong Anion, 60 mg / 3 mL, Tubes, 50/Pk
Condition 1 :3 mL Methanol
Condition 2 :3 mL 25 mM Ammonium Bicarbonate
Load :Load 0.50 mL Sample diluted in 25 mM Ammonium Bicarbonate
Wash 1 :3 mL 25mm ammonium bicarbonate buffer
Wash 2 :3 mL 25% methanol in water
Dry :5 min at high vacuum
Elution 1 :1 mL Ethyl Acetate:Isopropanol:NH4OH (7:2:1)
Elution 2 :1 mL Ethyl Acetate:Isopropanol:NH4OH (7:2:1)
Evaporate: :Evaporate to dryness
Reconstitute :0 µL initial mobile phase

应用详细信息 (应用ID: 20648)

货号:00B-4496-Y0
尺寸:50 x 3 mm ID
洗脱类型:Gradient
洗脱A:Water
洗脱B:50/50 AcCN/Methanol
梯度程序:
步骤序号 时间 (分) 图片A 图片B
1 0 70 30
2 4 10 90
3 5 10 90
4 5.1 70 30
5 7 70 30

流速 (毫升/分钟):0.5 mL/min
冷却温度: 室温
检测: Tandem Mass Spec (MS-MS) @ (22°C)

分析物详细信息

分析物详细信息不可用

1
Aldosterone
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