Region

应用

Histamine by LCMS using Luna 3um HILIC 100x2.1mm

样品制备详细信息

Part Number :8B-S035-TBJ
Description :Strata-X-CW 33 µm Polymeric Weak Cation, 30 mg / 3 mL, Tubes, 50/Pk
Condition 1 :1 mL Methanol
Condition 2 :1 mL DI water
Load :Load Sample
Wash 1 :2 mL DI water
Wash 2 :2 mL MeOH
Dry :2 min at high vacuum level, ~20-30 in Hg
Elution 1 :0.5 mL 87:13 B:A, fortified to contain 5% formic acid
Evaporate: :Evaporate to dryness
Reconstitute :0 µL initial mobile phase

应用详细信息 (应用ID: 20650)

货号:00D-4449-B0
尺寸:100 x 2 mm ID
洗脱类型:Gradient
洗脱A:50:45:5 ACN:DI H2O: 100 mM Amm. Form, pH 3.22
洗脱B:95:5 ACN:100 mM Amm. Formate, pH 3.22
梯度程序:
步骤序号 时间 (分) 图片A 图片B
1 0 17 83
2 1.5 100 0
3 2.5 100 0
4 2.6 17 83
5 4.5 17 83

流速 (毫升/分钟):0.6 mL/min
冷却温度: 室温
检测: Tandem Mass Spec (MS-MS) @ (22°C)
检测器信息: SCIEX API 5000™

分析物详细信息

分析物详细信息不可用

1
1-Methyl histamine
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2
N-Methyl histamine
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3
Histamine
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