Region

应用

Nicotine and metabolites by LCMS using Gemini NX 3u C18 100x2.1mm

样品制备详细信息

Part Number :8B-S029-UBJ
Description :Strata-X-C 33 µm Polymeric Strong Cation, 60 mg / 3 mL, Tubes, 50/Pk
Condition 1 :2 mL Methanol
Condition 2 :2 mL Ammonium acetate buffer
Load :Load Sample
Wash 1 :2 mL Ammonium acetate buffer
Wash 2 :2 mL 30% Methanol
Dry :10" Hg for 5 min
Elution 1 :2 mL 1.5% Ammonium hydroxide methanol solution
Elution 2 :2 mL 1.5% Ammonium hydroxide methanol solution
Evaporate: :Evaporate to dryness
Reconstitute :0 µL initial mobile phase

应用详细信息 (应用ID: 20651)

货号:00D-4453-B0
尺寸:100 x 2 mm ID
洗脱类型:Gradient
洗脱A:20mM Ammonium Bicarbonate
洗脱B:Acetonitrile
梯度程序:
步骤序号 时间 (分) 图片A 图片B
1 0 90 10
2 3 25 75
3 3.1 90 10
4 5 90 10

流速 (毫升/分钟):0.5 mL/min
冷却温度: 室温
检测: Tandem Mass Spec (MS-MS) @ (22°C)
检测器信息: SCIEX API 4000™

分析物详细信息

分析物详细信息不可用

1
Nornicotine
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2
3-Hydroxycotinine
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3
Anabasine
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4
Cotinine
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5
Nicotine
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