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应用

Testosterone and 17-OH-Progesterone by LCMS using Gemini NX 3u C18 50x2.1mm

样品制备详细信息

Part Number :8B-S123-TAK
Description :Strata-X-A 33 µm Polymeric Strong Anion, 30 mg / 1 mL, Tubes, 100/Pk
Condition 1 :1 mL Methanol
Condition 2 :1 mL Water
Load :Load Sample
Wash 1 :0.6 mL 50% MeOH
Dry :high vacuum for 2-3 min
Elution 1 :0.6 mL 100% MeOH
Elution 2 :0.6 mL 100% MeOH
Evaporate: :Evaporate to dryness
Reconstitute :0 µL initial mobile phase

应用详细信息 (应用ID: 20652)

货号:00B-4453-B0
尺寸:50 x 2 mm ID
洗脱类型:Gradient
洗脱A:Water with 0.1% formic acid
洗脱B:Acetonitrile with 0.1% formic acid
梯度程序:
步骤序号 时间 (分) 图片A 图片B
1 0 80 20
2 2.5 25 75
3 4.5 25 75
4 4.6 80 20
5 6 80 20

流速 (毫升/分钟):0.4 mL/min
冷却温度: 室温
检测: Tandem Mass Spec (MS-MS) @ (22°C)
检测器信息: SCIEX API 4000™

分析物详细信息

分析物详细信息不可用

1
Testosterone
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2
17-Hydroxyprogesterone
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