A Selective Method for Quantitation of Underivatized Methylmalonic Acid (MMA) in Plasma

Abstract:

In this technical note, we present a fast, reproducible LC/MS/MS method to analyze underivatized MMA by utilizing a unique Luna® Omega 1.6 μm PS C18. The method runtime is 5 minutes including column re-equilibration. For sample preparation procedure we used Strata®-X-AW solid phase extraction to produce a clean sample from plasma. Analyte detection was performed using negative mode electrospray ionization of a triple quadrupole MS.

Document Type:

Technical Notes

Industries:

Clinical

Techniques:

HPLC / UHPLC

Brands:

Luna Strata-X

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