A Unified Sample Preparation Procedure for General Unknown Screening (GUS) of Compounds in Whole Blood Samples

Abstract:

The identification of drugs from whole blood by high resolution mass spectrometer serves as an effective screening tool in both clinical and forensic labs. In addition, whole blood presents a challenging matrix that often requires an elaborate sample preparation procedure. The objectives of this work was to: • Develop a universal sample preparation procedure for whole blood samples to: lyse the erythrocytes, precipitate the majority of plasma proteins, and remove high levels of phopholipids present in the sample • Neutralize the undesirable effect of strong solvent

Document Type:

Technical Notes

Industries:

Clinical Forensics/Toxicology

Techniques:

HPLC / UHPLC Sample Prep

Brands:

Kinetex Phree

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