Beta-Glucuronidase Removal from Hydrolyzed Pain Management Urine Samples (TN-0083)

Abstract:

The escalating abuse of pain medicines has mandated that health care providers and practitioners routinely perform urine drug testing. Most laboratories opt for a dilute-and-shoot approach, along with enzymatic hydrolysis that requires no sample cleanup. The addition of extra protein as a result of hydrolysis using ß-glucuronidase to the urine can result in fouling of the LC column and loss of productivity. In this work, we present an automated method that uses an Impact™ Protein Precipitation 96-well plate to remove a majority of the proteins from hydrolyzed urine.

Document Type:

Technical Notes

Industries:

Forensics/Toxicology Clinical

Techniques:

Sample Prep HPLC / UHPLC

Brands:

Impact Kinetex

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