Effect of Perfluorinated Alcohols in Impurity Analysis for Synthetic Oligonucleotides

Abstract:

chromatographic performance is often improved by decreasing HFIP concentration, with acceptable or even optimal chromatography being obtained at 12.5 mM. Even more compelling is the improved ionization with the decrease in HFIP yielding significantly higher peak heights, facilitating characterization and quantitation.

Document Type:

Technical Notes

Industries:

Pharmaceutical/Biopharmaceutical

Techniques:

HPLC / UHPLC

Brands:

bioZen

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