Effect of Temperature on Single Stranded Oligonucleotide Analysis

Abstract:

Temperature is often utilized for oligonucleotide analysis. However, for single stranded oligos, there may not be a benefit to running at temperatures exceeding 60 °, as increases in temperature may not improve chromatography nor effect selectivity as one might observe with other macromolecules.

Document Type:

Technical Notes

Industries:

Pharmaceutical/Biopharmaceutical

Techniques:

HPLC / UHPLC

Brands:

bioZen

PDF Link:

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