Impact of pH on Improving Purity and Yield for Preparative Separations (TN-1050)

Abstract:

Increase yield and fraction purity for basic compounds using Gemini-NX preparative columns. By neutralizing the charge on basic compounds under high pH mobile phase, resolution and retention are significantly improved. Baseline resolution can be maintained even after increasing sample concentration 10x

Document Type:

Technical Notes

Industries:

Pharmaceutical/Biopharmaceutical

Techniques:

Preparative HPLC / UHPLC

Brands:

Axia Gemini

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