Native MS for Aggregate and Charge Variant Analysis for Biotherapeutics

Abstract:

Size exclusion chromatography (SEC) and ion exchange chromatography (IEX) are classic techniques employed in the characterization of biotherapeutics under nondenaturing conditions. Standard mobile-phase conditions for these techniques are high in salt concentration, rendering them incompatible with mass spectrometry (MS) detection. Native MS represents a cutting-edge approach to employ MS-compatible mobile phases for non-denaturing SEC and IEX analysis. Learn about aggregate and charge-variant analysis using native-mode MS, including understanding the fundamentals in SEC and IEX for biotherapeutic characterization, the effect of mobilephase conditions and chromatographic parameters in aggregate analysis by native SEC, and determination of acidic and basic variants of monoclonal antibodies using native weak cation exchange chromatography (WCX).

Document Type:

Supporting Docs

Industries:

Pharmaceutical/Biopharmaceutical

Techniques:

HPLC / UHPLC

Brands:

bioZen

PDF Link:

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