Nicotine, Cotinine, 3-Hydroxycotinine, Nornicotine and Anabasine in Human Urine by LC/MS/MS (TN-1161)
- Abstract:
- A method is illustrated for simultaneous analysis of nicotine, two of its major metabolites, cotinine and 3-hydroxycotinine, as well as nornicotine and anabasine from human urine samples. The method described uses Strata™-X-C solid phase extraction (SPE) cartridges for sample clean-up and concentration, followed by fast (<6 min) LC/MS/MS analysis using a Gemini® NX-C18 column.
- Document Type:
- Technical Notes
- Industries:
- Clinical
- Techniques:
- Sample Prep HPLC / UHPLC
- Brands:
- Strata-X Gemini
- PDF Link:
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