Optimization of a Gradient Profile on Reversed Phase Separations of Monoclonal Antibodies

Abstract:

The analysis of intact monoclonal antibodies (mAbs) by reversed phase HPLC is a common technique for assessing protein variation, such as clipping and heterogeneity due to post-translational modifications. Whether for LC-UV impurity analysis or for confirming primary sequence using LC-MS, the analysis of intact mAbs can provide useful insight on a protein therapeutic.

Document Type:

Technical Notes

Industries:

Pharmaceutical/Biopharmaceutical

Techniques:

HPLC / UHPLC

Brands:

bioZen

PDF Link:

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