Peptide Purification Method Development: Application for the Purification of Bivalirudin

Abstract:

Several reversed phase (RP) sorbents were tested in combination with various chromatographic conditions for the purification of Bivalirudin, a fairly hydrophilic peptide that elutes at 27 % of Acetonitrile on a C18 bonded sorbent. The crude peptide was synthesized by solid phase peptide synthesis (SPPS) and after TFA cleavage the crude peptide was obtained with a crude purity of 71.3 % .

Document Type:

Technical Notes

Industries:

Pharmaceutical/Biopharmaceutical

Techniques:

HPLC / UHPLC

Brands:

Luna

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