Optimization of Wash/Elution Conditions for Automated SPE of Testosterone from Plasma (TN-0017)

Abstract:

By providing additional interactive features such as pi-pi and hydrogen bonding interactions, Strata™-X enhances the retention of testosterone and sustains a 70% methanol wash. In contrast, the silica-based Strata® C18-E can tolerate only a 40 % methanol wash without loss of analyte. Such a wash is necessary to eliminate phospholipids and minimize ion suppression and other matrix effects, thereby improving analytical accuracy and precision.

Document Type:

Technical Notes

Industries:

Clinical

Techniques:

HPLC / UHPLC Sample Prep

Brands:

Gemini Strata-X

PDF Link:

Click here