Improving Selectivity for Intact Mass Spectrometry of Biotherapeutic mAbs

Abstract:

An often-overlooked consideration of intact MS analysis is chromatographic media selection. Subtle changes in selectivity are likely to occur, even with columns of the similar particle morphology and stationary phase (e.g. –C4) mainly due to differences in pore size distribution and surface chemistry. To demonstrate these differences, we tested two columns with a core-shell particle and butyl stationary phase

Document Type:

Technical Notes

Industries:

Pharmaceutical/Biopharmaceutical

Techniques:

HPLC / UHPLC

Brands:

bioZen

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